The BD Pharmingen™ Transcription Factor Buffer Set is optimized for fixing and permeabilizing cells prior to immunofluorescent staining and flow cytometric analysis of cells that express specific intracytoplasmic and intranuclear proteins. The BD Pharmingen™ Transcription Factor Buffer Set was designed to improve ease-of-use and minimize processing time, to reduce nonspecific staining, to increase the resolution of positively stained cells and to significantly reduce cell loss during fixation, permeabilization and staining procedures. Flow cytometric detection of the many proteins known to be expressed within various intracellular compartments, especially transcription factors, is improved with BD Pharmingen™ Transcription Factor Buffer Set use. This buffer system has been found useful for fixing and permeabilizing a variety of cell types from diverse human and mouse tissues. The buffer system is flexible in supporting multiwell-plate high-throughput and bulk sample analyses and applications that require overnight sample storage. The buffer system has minimal impact on the light-scatter and autofluorescence characteristics of processed cells resulting in characteristics similar to those observed for freshly prepared, highly viable primary cells. In many cases the buffer system was found to be compatible with the immunofluorescent staining of cell-surface antigens both before and after cellular fixation and permeabilization. The buffer system is also compatible with many tandem fluorochromes.

562574-BD Pharmingen™转录因子缓冲液套装 现货

562574-BD Pharmingen™转录因子缓冲液套装 现货

562574-BD Pharmingen™转录因子缓冲液套装 现货

BD Pharmingen™转录因子缓冲液套装优化用于在免疫荧光染色和表达特定胞质内和核内蛋白的细胞的流式细胞术分析之前固定和透化细胞。

产品介绍：

细胞内染色方案

1.固定：细胞表面染色程序完成后，吸出残留的染色缓冲液并通过短暂涡旋松开细胞沉淀。向每个管中加入1mL新鲜制备的1x Fix / Perm缓冲液工作溶液，并通过涡旋振荡约3秒重悬细胞沉淀。将样品在2-8°C孵育40-50分钟，避光。

2洗涤：将1ml 1x Perm /洗涤缓冲液直接加入悬浮在1x Fix / Perm缓冲液中的固定和透化细胞中。离心沉淀细胞。（注意：Fix / Perm后的所有离心步骤均为350 g ，在2-8°C下进行6分钟）。倾倒或吸出上清液。

3.洗涤：向沉淀的细胞中加入2ml 1x Perm /洗涤缓冲液，然后离心。倾析或吸出洗涤缓冲液。

4.细胞内染色：向细胞样品中加入80-100μl的1x Perm / Wash Buffer和对细胞内蛋白质特异的荧光抗体（如FoxP3，T-bet和/或IL-17A）和非特异性对照染色（例如，匹配荧光Ig同种型对照）到每个管。涡旋管或支架10秒钟，在2-8°C温育40-50分钟，避光。

5洗涤：在洗涤之前简单地涡旋样品。用2ml 1x Perm /洗涤缓冲液洗涤细胞。离心细胞。倾倒或吸出洗涤缓冲液。

6.洗涤：用2ml 1x Perm / Wash洗涤细胞。离心细胞。倾析或吸出洗涤缓冲液。

7.用于流式细胞术的样品制备：将细胞沉淀重悬于350μl流式细胞术染色缓冲液中。使用流式细胞仪分析细胞并获取数据。

562574-BD Pharmingen™转录因子缓冲液套装 现货

562574-BD Pharmingen™转录因子缓冲液套装 现货

562574-BD Pharmingen™转录因子缓冲液套装 现货