测序原始样本来源丰富,可分为DNA和RNA测序,下面进行DNA测序文库制备试剂盒的区分和选择进行对比:
可分为TruSeq和Nextera
相关产品如下:
品牌 | 货号 | 产品名称 | 包装量 | 产品特点 |
Illumina | IP-202-1012 | TruSeq ChIP Library Preparation | 12 indexes, 48 Rxns | ChIP来源DNA染色质免疫沉淀测序文库制备 |
Illumina | 20020614 | TruSeq DNA Exome | 24 样本 | 外显子组文库制备 |
Illumina | 20015964 | TruSeq Nano DNA Low Throughput Library Prep Kit | 24 samples | 100-200ng起始DNA,6h操作时间 |
Illumina | 20015965 | TruSeq Nano DNA High Throughput Library Prep Kit | 96 samples | |
Illumina | 20015962 | TruSeq DNA PCR-Free Low Throughput Library Prep Kit | 24 samples | 1-2ug起始 |
Illumina | 20015963 | TruSeq DNA PCR-Free High Throughput Library Prep Kit | 96 samples | |
Illumina | FC-121-1030/1031 | Nextera DNA Library Prep Kit | 24/96 samples | 50ng 起始 |
Illumina | FC-131-1024 | Nextera XT DNA Library Preparation Kit | 24 samples | 1ng 起始,耗时1.5h |
Illumina | FC-131-1096 | Nextera XT DNA Library Preparation Kit | 96 samples |
总结:
TruSeq DNA建库方法复杂,文库起始量500ng,对DNA质量要求较低,成本较低,基因组覆盖度较高。------------采用超声波方法对DNA进行随机打断,然后加接头,片段筛选和PCR扩增
Nextera DNA建库方法简单,文库起始量50ng,对DNA质量要求较高,成本较高,基因组覆盖度较低。------------采用转座酶随机插入并将基因组DNA打断成长度大小为300bp左右的片段,同时将测序所需的adaptor直接在插入打断的同时连接到片段的两端
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